Biomedical materials have different optical properties (e.g. absorption) for different wavelengths. Therefore, probing biomedical materials with multiple wavelengths not only can get in-depth understanding of the detected biomedical materials but also can differentiate the detected biomedical materials. To achieve this purpose, in this conference paper, we present our initial results of building up a portable multiple-wavelength biomedical sensing system. At this initial phase, we assembled this kind of system with multiple wavelengths of light sources and photodetectors and preliminarily tested the absorbance of the glucose solutions with different concentrations. The result shows good linearity of the absorbance of the glucose solution with concentration. In addition, we also measured the absorbance of the glucose solutions using a broadband white light source and a spectrometer. This result also exhibits linearity but different slop of absorbance with glucose concentration, which confirms the linearity result obtained from the built sensing system. The difference of slop maybe relates to the difference of optical design between these two systems.