TY - JOUR
T1 - Unique formosan mushroom Antrodia camphorata differentially inhibits androgen-responsive LNCaP and -independent PC-3 prostate cancer cells
AU - Chen, Kuan Chou
AU - Peng, Chiung Chi
AU - Peng, Robert Y.
AU - Su, Ching Hua
AU - Chiang, Han Sun
AU - Yan, Jr Hung
AU - Hsieh-Li, Hsiu Mei
N1 - Funding Information:
We owe many thanks to Ms. Tzu-Wen Tsai for her valuable technical assistance. This work was supported in part by the research grant NSC-93–2320-B-003-006 from the National Science Council, Taiwan. Address correspondence to H. M. Hsieh-Li, Department of Life Science, National Taiwan Normal University, 88, Sec. 4, Ting-Chou Road, Taipei 116, Taiwan. Phone: +886-2-29336876×214. FAX: +886-2-29312904. E-mail: [email protected].
PY - 2007
Y1 - 2007
N2 - Antrodia camphorata (AC), a precious and unique folkloric medicinal mushroom enriched in polyphenolics, isoflavonoids, triterpenoids, and polysaccharides, has been diversely used in Formosa (Taiwan) since the 18th century. In this study, prostate cancer (PCa) cell lines PC-3 (androgen independent) and LNCaP (androgen responsive) were treated with AC crude extract (ACCE) at 50-200 μg/mL, respectively, for 48 h. At the minimum effective dose 150 μg/mL, LNCaP showed a G1/S phase arrest with significant apoptosis. Such dose-dependent behavior of LNCaP cells in response to ACCE was confirmed to proceed as Akt → p53 → p21 → CDK4/cyclin D1 → G1/S-phase arrest → apoptosis, which involved inhibiting cyclin D1 activity and preventing pRb phosphorylation. In contrast, being without p53, PC-3 cells showed a G2/M-phase arrest mediated through pathway p21 → cyclin B1/Cdc2 → G2/M-phase arrest, however, with limited degree of apoptosis, implicating that ACCE is able to differentially inhibit the growth of different PCa cells by modulating different cell cycle signaling pathways. We conclude that this unique Formosan mushroom, A. camphorata, due to its nontoxicity, might be used as a good adjuvant anticancer therapy for prostate cancers despite its androgen-responsive behaviors, which has long been a serious drawback often encountered clinically in hormonal refractory cases treated by antihormonal therapies and chemotherapeutics.
AB - Antrodia camphorata (AC), a precious and unique folkloric medicinal mushroom enriched in polyphenolics, isoflavonoids, triterpenoids, and polysaccharides, has been diversely used in Formosa (Taiwan) since the 18th century. In this study, prostate cancer (PCa) cell lines PC-3 (androgen independent) and LNCaP (androgen responsive) were treated with AC crude extract (ACCE) at 50-200 μg/mL, respectively, for 48 h. At the minimum effective dose 150 μg/mL, LNCaP showed a G1/S phase arrest with significant apoptosis. Such dose-dependent behavior of LNCaP cells in response to ACCE was confirmed to proceed as Akt → p53 → p21 → CDK4/cyclin D1 → G1/S-phase arrest → apoptosis, which involved inhibiting cyclin D1 activity and preventing pRb phosphorylation. In contrast, being without p53, PC-3 cells showed a G2/M-phase arrest mediated through pathway p21 → cyclin B1/Cdc2 → G2/M-phase arrest, however, with limited degree of apoptosis, implicating that ACCE is able to differentially inhibit the growth of different PCa cells by modulating different cell cycle signaling pathways. We conclude that this unique Formosan mushroom, A. camphorata, due to its nontoxicity, might be used as a good adjuvant anticancer therapy for prostate cancers despite its androgen-responsive behaviors, which has long been a serious drawback often encountered clinically in hormonal refractory cases treated by antihormonal therapies and chemotherapeutics.
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U2 - 10.1080/01635580701268360
DO - 10.1080/01635580701268360
M3 - Article
C2 - 17516868
AN - SCOPUS:34249888778
SN - 0163-5581
VL - 57
SP - 111
EP - 121
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 1
ER -
