Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor

C. C. Chang, S. H. Lin, N. F. Chiu, C. W. Lin

    研究成果: 書貢獻/報告類型會議貢獻

    摘要

    In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. CA15.3 tumor marker for breast cancer was chosen as a model analyte. We analyzed experimentally intensity response to the samples that had different concentrations (0.0125 U/ml to 160 U/ml) in pleural fluid to evaluate the detection capability of the Au/ZnO SPR-based biosensor. As a result, it was found that the linear range extended from 0.025 to 40 U/ml with correlation coefficient of R = 0.9531 and the limit of detection at S/N of 3:1 reached 0.025U/ml. Compared with the shift degree of SPR intensity by the specific binding event between antibody and antigen, the change of intensity on the Au/ZnO layers increased at least 2 fold more than that on the Au/Cr layers. In addition, we determined that the Au/ZnO layers had a detection limit 4 times lower than the Au/Cr layers that were widespread use of the sensing interfaces on SPR. In conclusion, the use of Au/ZnO films greatly enhanced the SPR signal after carrying out bimolecular interactions and presented high sensitivity and reproducibility.

    原文英語
    主出版物標題Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
    頁面172-175
    頁數4
    2
    出版狀態已發佈 - 2009 十二月 1
    事件Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, 美国
    持續時間: 2009 五月 32009 五月 7

    其他

    其他Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
    國家美国
    城市Houston, TX
    期間09/5/309/5/7

      指紋

    ASJC Scopus subject areas

    • Biotechnology
    • Biomedical Engineering
    • Biomaterials

    引用此

    Chang, C. C., Lin, S. H., Chiu, N. F., & Lin, C. W. (2009). Ultrasensitive protein detection using an Au/ZnO SPR-based biosensor. 於 Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (卷 2, 頁 172-175)