Ultrasensitive detection of nanoparticle-based bio bar-code DNA sensor by using nano-gap electrodes

Tien Li Chang, Chien Ying Tsai, Ramesh Uppala, Chun Chi Chen, Chun Hung Lin, Ping Hei Chen

研究成果: 書貢獻/報告類型會議貢獻

摘要

An ultra sensitive approach to detect the sub femtomolar DNA by using electrical detection method on a DNA biochip has been developed. The system provides high selectivity with sensitivity which is comparable to nano-gap-electrode detection approaches without the need of PCR amplification. Because bio-bar-code DNA approach is a pseudohomogeneous system with both magnetic nanoparticles and gold nanoparticles in solution, low concentration of the probes can be used very efficiently to bind target DNA, thereby reducing time required for high sensitivity detection experiments. Indeed, an advantage of the bio-bar-code DNA approach over conventional microarray sandwich assembly assays is that the entire self-assembly assay can be carried out in sub femtomolar concentration, regardless of target concentration. The system has an excellent dynamic range and is ideally set up for multiplexing.

原文英語
主出版物標題Digest of Papers - Microprocesses and Nanotechnology 2005
主出版物子標題2005 International Microprocesses and Nanotechnology Conference
頁面210-211
頁數2
2005
出版狀態已發佈 - 2005
對外發佈Yes
事件2005 International Microprocesses and Nanotechnology Conference - Tokyo, 日本
持續時間: 2005 十月 252005 十月 28

其他

其他2005 International Microprocesses and Nanotechnology Conference
國家日本
城市Tokyo
期間05/10/2505/10/28

    指紋

ASJC Scopus subject areas

  • Engineering(all)

引用此

Chang, T. L., Tsai, C. Y., Uppala, R., Chen, C. C., Lin, C. H., & Chen, P. H. (2005). Ultrasensitive detection of nanoparticle-based bio bar-code DNA sensor by using nano-gap electrodes. 於 Digest of Papers - Microprocesses and Nanotechnology 2005: 2005 International Microprocesses and Nanotechnology Conference (卷 2005, 頁 210-211). [1595288]