Ultrasensitive Detection of Alzheimer's Amyloids on a Plasmonic-Gold Platform

Hsiao Fen Han, Hung Chi Yen, Hsiu Chuan Wu, Hsin Yuan Tan, Wei Xu, Hang Shiang Jiang, Ping Jui Tsai, Kun Qian, Yi Chun Wu*, Chia Chun Chen

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

More than 55 million people live with dementia worldwide in 2021, and there are nearly 10 million new cases every year. Alzheimer's disease (AD) is the most common cause of dementia. Despite urgent need, early detection of AD and long-term monitoring of AD progression have been challenging. This is due to the limited availability of brain imaging facilities and the highly invasive procedure with the cerebrospinal fluid assay to assess the level of AD biomarkers, such as beta-amyloid (Aβ). Reliable measurements of AD biomarkers in blood samples are still difficult because of their very low abundance. Here, we develop a rapid, specific, and ultrasensitive immunoassay using plasmonic-gold nanoisland (pGOLD) chips with near-infrared fluorescence-enhanced detection for Aβ1-40 and Aβ1-42. We show step-by-step processes and results during the platform establishment, including antibody specificity and sensitivity tests, antibody pair examination, condition optimization, and procedure refinement. Finally, we demonstrate the platform performance with detection sensitivity at the subpicogram per milliliter level. This platform, therefore, has a great application potential for early detection of AD using blood samples.

原文英語
頁(從 - 到)57036-57042
頁數7
期刊ACS Applied Materials and Interfaces
13
發行號48
DOIs
出版狀態已發佈 - 2021 12月 8

ASJC Scopus subject areas

  • 材料科學(全部)

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