We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at ∼-70°C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.
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