Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine

Cheng You Liu; Yi Chieh Chou; Jui Hsuan Tsai; Tzu Ming Huang; Jian Zhang Chen; Yi Chun Yeh

doi:10.3390/app9040622

Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine

Cheng You Liu, Yi Chieh Chou, Jui Hsuan Tsai, Tzu Ming Huang, Jian Zhang Chen^*, Yi Chun Yeh

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

28 引文斯高帕斯（Scopus）

摘要

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 μM and 40-500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

原文	英語
文章編號	622
期刊	Applied Sciences (Switzerland)
卷	9
發行號	4
DOIs	https://doi.org/10.3390/app9040622
出版狀態	已發佈 - 2019 2月 13

ASJC Scopus subject areas

一般材料科學
儀器
一般工程
製程化學與技術
電腦科學應用
流體流動和轉移過程

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10.3390/app9040622

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title = "Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine",

abstract = "Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 μM and 40-500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.",

keywords = "Chitosan, Dopamine, Electrochemical biosensor, Reduced graphene oxide, Screen printing, Tyrosinase",

author = "Liu, {Cheng You} and Chou, {Yi Chieh} and Tsai, {Jui Hsuan} and Huang, {Tzu Ming} and Chen, {Jian Zhang} and Yeh, {Yi Chun}",

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T1 - Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine

AU - Liu, Cheng You

AU - Chou, Yi Chieh

AU - Tsai, Jui Hsuan

AU - Huang, Tzu Ming

AU - Chen, Jian Zhang

AU - Yeh, Yi Chun

PY - 2019/2/13

Y1 - 2019/2/13

N2 - Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 μM and 40-500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

AB - Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 μM and 40-500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

KW - Chitosan

KW - Dopamine

KW - Electrochemical biosensor

KW - Reduced graphene oxide

KW - Screen printing

KW - Tyrosinase

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Tyrosinase/Chitosan/Reduced graphene oxide modified screen-printed carbon electrode for sensitive and interference-free detection of dopamine

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ASJC Scopus subject areas

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