Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih, Sheng Han Lo, Ni Shin Yang, Brenda Singco, Yi Jie Cheng, Cheng You Wu, I. Hsin Chang, Hsi Ya Huang*, Chia Her Lin

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

146 引文 斯高帕斯(Scopus)

摘要

Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

原文英語
頁(從 - 到)982-986
頁數5
期刊ChemPlusChem
77
發行號11
DOIs
出版狀態已發佈 - 2012 11月
對外發佈

ASJC Scopus subject areas

  • 一般化學

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