Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih; Sheng Han Lo; Ni Shin Yang; Brenda Singco; Yi Jie Cheng; Cheng You Wu; I. Hsin Chang; Hsi Ya Huang; Chia Her Lin

doi:10.1002/cplu.201200186

Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

Yung Han Shih, Sheng Han Lo, Ni Shin Yang, Brenda Singco, Yi Jie Cheng, Cheng You Wu, I. Hsin Chang, Hsi Ya Huang^*, Chia Her Lin

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研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

112 引文斯高帕斯（Scopus）

摘要

Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

原文	英語
頁（從 - 到）	982-986
頁數	5
期刊	ChemPlusChem
卷	77
發行號	11
DOIs	https://doi.org/10.1002/cplu.201200186
出版狀態	已發佈 - 2012 11月
對外發佈	是

ASJC Scopus subject areas

化學 (全部)

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10.1002/cplu.201200186

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abstract = "Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.",

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author = "Shih, {Yung Han} and Lo, {Sheng Han} and Yang, {Ni Shin} and Brenda Singco and Cheng, {Yi Jie} and Wu, {Cheng You} and Chang, {I. Hsin} and Huang, {Hsi Ya} and Lin, {Chia Her}",

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doi = "10.1002/cplu.201200186",

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AU - Shih, Yung Han

AU - Lo, Sheng Han

AU - Yang, Ni Shin

AU - Singco, Brenda

AU - Cheng, Yi Jie

AU - Wu, Cheng You

AU - Chang, I. Hsin

AU - Huang, Hsi Ya

AU - Lin, Chia Her

PY - 2012/11

Y1 - 2012/11

N2 - Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

AB - Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal-organic frameworks (MOFs). After separation by nano-LCMS2 (liquid chromatography-mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

KW - Enzyme immobilization

KW - Mass spectrometry

KW - Metal-organic framework

KW - Nanobiocatalysis

KW - Proteomics

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Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis

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