Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

S. H. Liao; H. C. Yang; H. E. Horng; J. J. Chieh; K. L. Chen; H. H. Chen; J. Y. Chen; C. I. Liu; C. W. Liu; L. M. Wang

doi:10.1063/1.4846536

Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

S. H. Liao^*, H. C. Yang, H. E. Horng, J. J. Chieh, K. L. Chen, H. H. Chen, J. Y. Chen, C. I. Liu, C. W. Liu, L. M. Wang

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

19 引文斯高帕斯（Scopus）

摘要

In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe ₃O₄, labeled as Fe₃O₄-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe ₃O₄-antiCRP, the magnetic clusters of Fe₃O ₄-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

原文	英語
文章編號	243703
期刊	Applied Physics Letters
卷	103
發行號	24
DOIs	https://doi.org/10.1063/1.4846536
出版狀態	已發佈 - 2013 12月 9

ASJC Scopus subject areas

物理與天文學（雜項）

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10.1063/1.4846536

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Liao, S. H., Yang, H. C., Horng, H. E., Chieh, J. J., Chen, K. L., Chen, H. H., Chen, J. Y., Liu, C. I., Liu, C. W., & Wang, L. M. (2013). Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays. Applied Physics Letters, 103(24), 文章 243703. https://doi.org/10.1063/1.4846536

Liao, SH, Yang, HC, Horng, HE , Chieh, JJ, Chen, KL, Chen, HH, Chen, JY, Liu, CI, Liu, CW & Wang, LM 2013, 'Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays', Applied Physics Letters, 卷 103, 編號 24, 243703. https://doi.org/10.1063/1.4846536

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title = "Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays",

abstract = "In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.",

author = "Liao, {S. H.} and Yang, {H. C.} and Horng, {H. E.} and Chieh, {J. J.} and Chen, {K. L.} and Chen, {H. H.} and Chen, {J. Y.} and Liu, {C. I.} and Liu, {C. W.} and Wang, {L. M.}",

note = "Funding Information: This work was supported by National Science Council of Taiwan under Grant Nos. 101-2811-M-168-001, 102-2120-M-168-001, 102-2112-M-168-001, 102-2112-M-003-017, 101-2112-M-003-009, 102-2923-M003-001, 102-2622-M002-001-CC2, 102-2112-M-003-008-MY2, 100-2112-M-003-011-MY2 and 99-2112-M-168-001-MY3, the Ministry of Health and Welfare under Grant No. DOH102-TD-PB-111-TM007 and DOH102-TD-N-111-002.",

year = "2013",

month = dec,

day = "9",

doi = "10.1063/1.4846536",

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AU - Liao, S. H.

AU - Yang, H. C.

AU - Horng, H. E.

AU - Chieh, J. J.

AU - Chen, K. L.

AU - Chen, H. H.

AU - Chen, J. Y.

AU - Liu, C. I.

AU - Liu, C. W.

AU - Wang, L. M.

N1 - Funding Information: This work was supported by National Science Council of Taiwan under Grant Nos. 101-2811-M-168-001, 102-2120-M-168-001, 102-2112-M-168-001, 102-2112-M-003-017, 101-2112-M-003-009, 102-2923-M003-001, 102-2622-M002-001-CC2, 102-2112-M-003-008-MY2, 100-2112-M-003-011-MY2 and 99-2112-M-168-001-MY3, the Ministry of Health and Welfare under Grant No. DOH102-TD-PB-111-TM007 and DOH102-TD-N-111-002.

PY - 2013/12/9

Y1 - 2013/12/9

N2 - In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

AB - In this work, the time-dependent phase lag θ of biofunctionalized magnetic nanoparticles (BMNs) conjugated with biotargets is studied with a home-made alternating current (ac) susceptometor for liquid phase immunoassays. The sensing unit of the ac susceptometor composed of excitation, pick-up, and compensation coils are balanced to 0.03 ppm. The BMNs are anti-goat C-reactive protein coated onto dextran-coated magnetic nanoparticles composed of Fe 3O4, labeled as Fe3O4-antiCRP. The bio-targets are human CRP. As the human CRP is conjugated with reagents Fe 3O4-antiCRP, the magnetic clusters of Fe3O 4-antiCRP-CRP are formulated. Due to the clustering effect, the Brownian relaxation of BMNs will be depressed, which in turn enhances the effective relaxation time. By monitoring the dynamic phase lag, we demonstrate a sensitive platform of assaying human CRP. The detection platform is robust, easy to use and can be applied for assaying a wide variety of biotargets including viruses, proteins, tumor markers, chemicals, etc.

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ER -

Time-dependent phase lag of biofunctionalized magnetic nanoparticles conjugated with biotargets studied with alternating current magnetic susceptometor for liquid phase immunoassays

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