Three-dimensional super-resolution imaging of the cytoskeleton in hippocampal neurons using selective plane illumination

Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin Yi Chen, Chieh Han Lu, Wei Chun Tang, Yen Ting Liu, Bi Chang Chen*, Peilin Chen

*此作品的通信作者

研究成果: 書貢獻/報告類型篇章

摘要

Recent advances in single-molecule-based super-resolution imaging have been in the forefront of biological research for the visualization of the detail structures in cellular and molecular biology. A number of super-resolution optical microscopy techniques have been reported; however, several challenges such as the use of high-activation sources resulting in photobleaching and photodamage effects, restrictions in three-dimensional imaging, long data acquisition, and limited field of view remain unresolved. To address these concerns, a rapid, large-scale, and three-dimensional super-resolution fluorescence microscopy has been developed through the introduction of selective plane illumination microscopy based on scanning Bessel beam and a spontaneously blinking dye HMSiR as a reporter. This localization-based super-resolution microscope offers several advantages. namely minuscule levels of photodamage and phototoxicity effects due to low activation source, good optical sectioning suitable for three-dimensional imaging, large field of view, and fast data acquisition. In this chapter, protocols on the three-dimensional super-resolution imaging of neurons through the application of selective plane illumination technique are discussed.

原文英語
主出版物標題Neuromethods
發行者Humana Press Inc.
頁面261-293
頁數33
DOIs
出版狀態已發佈 - 2020
對外發佈

出版系列

名字Neuromethods
154
ISSN(列印)0893-2336
ISSN(電子)1940-6045

ASJC Scopus subject areas

  • 一般神經科學
  • 一般生物化學,遺傳學和分子生物學
  • 一般藥理學、毒理學和製藥學
  • 精神病學和心理健康

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