The solution structure of the active domain of CAP18 - a lipopolysaccharide binding protein from rabbit leukocytes

Chinpan Chen, Roland Brock, Frederick Luh, Ping Jung Chou, James W. Larrick, Rong Fong Huang, Tai huang Huang

    研究成果: 雜誌貢獻文章同行評審

    60 引文 斯高帕斯(Scopus)

    摘要

    We have employed the circular dichroism (CD) technique to characterize the solution structure of CAP18106-137, a lipopolysaccharide (LPS) binding, antimicrobial protein, and its interaction with lipid A. Our results revealed that CAP18106-137 may exist in at least three lipid A concentration-dependent, primarily helix conformations. The 'model' structure of CAP18106-137 in 30% (v/v) TFE, determined by nuclear magnetic resonance (NMR) technique, was found to be a complete and very rigid helix. In this conformation, the cationic and hydrophobic groups of CAP18106-137 are separated into patches and stripes in such a way that it can favorably interact with lipid A through either coulombic interaction with the diphosphoryl groups or hydrophobic interaction with the fatty acyl chains.

    原文英語
    頁(從 - 到)46-52
    頁數7
    期刊FEBS Letters
    370
    發行號1-2
    DOIs
    出版狀態已發佈 - 1995 八月 14

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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