The secondary structure of a pyrimidine-guanine sequence-specific ribonuclease possessing cytotoxic activity from the oocytes of Rana catesbeiana

Chinpan Chen, Kellie Hom, Rong Fong Huang, Ping Jung Chou, You Di Liao, Tai Huang Huang

研究成果: 雜誌貢獻期刊論文同行評審

7 引文 斯高帕斯(Scopus)

摘要

RC-RNase is a pyrimidine-guanine sequence-specific ribonuclease and a sialic-acid-binding lectin purified from Rana catesbeiana (bullfrog) oocytes. This 111-amino acid protein exhibits cytotoxicity toward several tumor cell lines. In this paper we report the assignments of proton NMR resonances and the identification of the secondary structure deduced from NOE constraints, chemical shift index, 3JNHα and amide proton exchange rates. The protein was directly isolated from bullfrog oocytes; we were able to assign all but five of the amino acid backbone protons of the unlabeled protein by analyzing a large set of two-dimensional proton NMR spectra obtained at several temperatures and pH conditions. Our results indicate that the structure of RC-RNase is dominated by the presence of two triple-stranded anti-parallel β-sheets and three α-helices, similar to those of the pyrimidine family ribonucleases. Two sets of resonances were observed for 11 amide protons and 8 α-protons located in the loop-1 region, an α2 helix, and three β-strands (β1, β3 and β4), suggesting the presence of nonlocalized multiple conformations for RC-RNase.

原文英語
頁(從 - 到)331-344
頁數14
期刊Journal of Biomolecular NMR
8
發行號3
DOIs
出版狀態已發佈 - 1996 一月 1

ASJC Scopus subject areas

  • 生物化學
  • 光譜

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