The Novel Heterocyclic Trioxirane [(1,3,5-Tris Oxiran-2-yl)Methyl)-1,3,5-Triazinane-2,4,6-Trione (TATT)] Exhibits a Better Anticancer Effect than Platinum-Based Chemotherapy by Induction of Apoptosis and Curcumin Further Enhances its Chemosensitivity

Chun Li Su*, Ying Ti Wang, Mu Hsin Chang, Kang Fang, Kwunmin Chen

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

4 引文 斯高帕斯(Scopus)

摘要

The heterocyclic trioxirane compound [1,3,5-tris((oxiran-2-yl)methyl)-1,3,5-triazinane-2,4,6-trione (TATT)] is a synthetic compound which has been used as an experimental anticancer agent in human clinical trials. Curcumin, an active natural compound in turmeric and curry, is an ingredient commonly used in the traditional diet of many Asian countries. In the present study, we observed that TATT exhibited a better anticancer effect on chemoresistant human colorectal cancer HT-29 cells and displayed less cytotoxicity on normal human umbilical vein endothelial cells, compared with FDA-approved anticancer drugs (cisplatin, carboplatin, or oxaliplatin) using MTT assay. TATT also induced a stronger apoptotic effect than that seen with the three studied anticancer drugs, as characterized by externalization of phosphatidylserine using flow cytometry. Administration of caspase 8-specific inhibitor (z-IETD-fmk) and mitochondrial permeability transition pore inhibitor (cyclosporin A) demonstrated that TATT-induced apoptosis proceeded via both extrinsic and intrinsic signaling pathways. It is noteworthy that coadministration of curcumin further significantly increased TATT-induced cytotoxicity, externalization of phosphatidylserine (representing early apoptosis), and the percentages of cells at the sub-G1 phase (representing late apoptosis), producing an additivity and/or synergistic effect, and vice versa. Suppression of nuclear NF-κB was involved in curcumin-enhanced chemosensitivity of TATT. Overall, our data indicate that TATT exerts a chemotherapeutic effect on colorectal cancer cells and coadministration of curcumin enhances the treatment effect of TATT.

原文英語
頁(從 - 到)597-609
頁數13
期刊Cell Biochemistry and Biophysics
68
發行號3
DOIs
出版狀態已發佈 - 2014 四月

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 細胞生物學

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