The hemocompatibility of oxidized diamond nanocrystals for biomedical applications

Hung Cheng Li, Feng Jen Hsieh, Ching Pin Chen, Ming Yao Chang, Patrick C.H. Hsieh, Chia Chun Chen, Shain Un Hung, Che Chih Wu, Huan Cheng Chang

研究成果: 雜誌貢獻期刊論文同行評審

28 引文 斯高帕斯(Scopus)

摘要

Low-dimensional carbon-based nanomaterials have recently received enormous attention for biomedical applications. However, increasing evidence indicates that they are cytotoxic and can cause inflammatory responses in the body. Here, we show that monocrystalline nanodiamonds (NDs) synthesized by high-pressure-high-temperature (HPHT) methods and purified by air oxidation and strong oxidative acid treatments have excellent hemocompatibility with negligible hemolytic and thrombogenic activities. Cell viability assays with human primary endothelial cells suggested that the oxidized HPHT-NDs (dimensions of 35-500 nm) are non-cytotoxic. No significant elevation of the inflammatory cytokine levels of IL-1β and IL-6 was detected in mice after intravenous injection of the nanocrystals in vivo. Using a hindlimb-ischemia mouse model, we demonstrated that 35-nm NDs after covalent conjugation with polyarginine are useful as a drug delivery vehicle of heparin for prolonged anticoagulation treatment. The present study lays a solid foundation for further therapeutic applications of NDs in biomedicine.

原文英語
文章編號3044
期刊Scientific Reports
3
DOIs
出版狀態已發佈 - 2013 十月 25
對外發佈Yes

ASJC Scopus subject areas

  • General

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