The Caulobacter Tol-Pal complex is essential for outer membrane integrity and the positioning of a polar localization factor

Yi Chun Yeh, Luis R. Comolli, Kenneth H. Downing, Lucy Shapiro, Harley H. McAdams

研究成果: 雜誌貢獻文章

62 引文 斯高帕斯(Scopus)

摘要

Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caulobacter cell envelope exhibited outer membrane disruption, and cells failed to complete cell division in TolA, TolB, or Pal mutant strains. In wild-type cells, components of the Tol-Pal complex localize to the division plane in early predivisional cells and remain predominantly at the new pole of swarmer and stalked progeny upon completion of division. The Tol-Pal complex is required to maintain the position of the transmembrane TipN polar marker, and indirectly the PleC histidine kinase, at the cell pole, but it is not required for the polar maintenance of other transmembrane and membrane-associated polar proteins tested. Coimmunoprecipitation experiments show that both TolA and Pal interact directly or indirectly with TipN. We propose that disruption of the trans-envelope Tol-Pal complex releases TipN from its subcellular position. The Caulobacter Tol-Pal complex is thus a key component of cell envelope structure and function, mediating OM constriction at the final step of cell division as well as the positioning of a protein localization factor.

原文英語
頁(從 - 到)4847-4858
頁數12
期刊Journal of Bacteriology
192
發行號19
DOIs
出版狀態已發佈 - 2010 十月 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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