The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Bao Shiang Lee; Huey Nan Wu; Tai Huang Huang

doi:10.1016/0014-5793(93)80138-K

The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

Bao Shiang Lee
, Huey Nan Wu
, Tai Huang Huang^*

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

6 引文斯高帕斯（Scopus）

摘要

We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

原文	英語
頁（從 - 到）	296-300
頁數	5
期刊	FEBS Letters
卷	324
發行號	3
DOIs	https://doi.org/10.1016/0014-5793(93)80138-K
出版狀態	已發佈 - 1993 6月 21
對外發佈	是

UN SDG

此研究成果有助於以下永續發展目標

SDG 3 健康與福祉

ASJC Scopus subject areas

生物物理學
結構生物學
生物化學
分子生物學
遺傳學
細胞生物學

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10.1016/0014-5793(93)80138-K

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title = "The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study",

abstract = "We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.",

keywords = "Hepatitis delta virus, NMR, RNA: Structure, Ribozyme",

author = "Lee, \{Bao Shiang\} and Wu, \{Huey Nan\} and Huang, \{Tai Huang\}",

note = "Funding Information: Ackrlo\textasciitilde{}\textasciitilde{}lrtigen?erzWtse thank Prof. B.C. Wang for the gtft of T7 RNA polymerase. Ms. Y.C. Lai and H.W. Hwang for asststancew tth sample preparation, and Ms. L.H. Tseng for asststance with NMR experiments. This work was supported by a grant from Nattonal Sctence Council of the Republic of Chma.",

year = "1993",

month = jun,

day = "21",

doi = "10.1016/0014-5793(93)80138-K",

language = "English",

volume = "324",

pages = "296--300",

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number = "3",

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TY - JOUR

T1 - The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

AU - Lee, Bao Shiang

AU - Wu, Huey Nan

AU - Huang, Tai Huang

N1 - Funding Information: Ackrlo~~lrtigen?erzWtse thank Prof. B.C. Wang for the gtft of T7 RNA polymerase. Ms. Y.C. Lai and H.W. Hwang for asststancew tth sample preparation, and Ms. L.H. Tseng for asststance with NMR experiments. This work was supported by a grant from Nattonal Sctence Council of the Republic of Chma.

PY - 1993/6/21

Y1 - 1993/6/21

N2 - We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

AB - We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32°C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 ± 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetraloop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.

KW - Hepatitis delta virus

KW - NMR

KW - RNA: Structure

KW - Ribozyme

UR - https://www.scopus.com/pages/publications/0027289655

UR - https://www.scopus.com/pages/publications/0027289655#tab=citedBy

U2 - 10.1016/0014-5793(93)80138-K

DO - 10.1016/0014-5793(93)80138-K

M3 - Article

C2 - 8405369

AN - SCOPUS:0027289655

SN - 0014-5793

VL - 324

SP - 296

EP - 300

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

The catalytic domain of human hepatitis delta virus RNA. A proton nuclear magnetic resonance study

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ASJC Scopus subject areas

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