Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

Chih Chin Shih; Chuan Chin Huang; Shou Hsien Li; Mei Hsiu Hwang; Ling Ling Lee

doi:10.1007/s10592-009-9830-3

Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

Chih Chin Shih
, Chuan Chin Huang
, Shou Hsien Li
, Mei Hsiu Hwang
, Ling Ling Lee^*

^*此作品的通信作者

生命科學系

研究成果: 雜誌貢獻 › 評論/辯論 › 同行評審

14 引文斯高帕斯（Scopus）

摘要

Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

原文	英語
頁（從 - 到）	1845-1847
頁數	3
期刊	Conservation Genetics
卷	10
發行號	6
DOIs	https://doi.org/10.1007/s10592-009-9830-3
出版狀態	已發佈 - 2009 11月

ASJC Scopus subject areas

生態學、進化論、行為學與系統學
遺傳學

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10.1007/s10592-009-9830-3

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title = "Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus",

abstract = "Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.",

keywords = "Formosan black bear, Tailed primers, Tetranucleotide microsatellite, Ursus thibetanus",

author = "Shih, \{Chih Chin\} and Huang, \{Chuan Chin\} and Li, \{Shou Hsien\} and Hwang, \{Mei Hsiu\} and Lee, \{Ling Ling\}",

note = "Funding Information: Acknowledgments We acknowledge Mr. Chieh-Chung Yang in Taiwan Endemic Species Research Institute for providing bear samples. We also thank Ms. Rong-Chien Lin in the Genetic Diversity Laboratory of National Taiwan Normal University for her help with the lab work. This study was funded by the Taiwan Forest Bureau, Council of Agriculture, ROC (95AS-11.1.3-FB-e1) to L-L Lee and supported by Taipei Zoo to C–C Shih.",

year = "2009",

month = nov,

doi = "10.1007/s10592-009-9830-3",

language = "English",

volume = "10",

pages = "1845--1847",

journal = "Conservation Genetics",

issn = "1566-0621",

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AU - Shih, Chih Chin

AU - Huang, Chuan Chin

AU - Li, Shou Hsien

AU - Hwang, Mei Hsiu

AU - Lee, Ling Ling

N1 - Funding Information: Acknowledgments We acknowledge Mr. Chieh-Chung Yang in Taiwan Endemic Species Research Institute for providing bear samples. We also thank Ms. Rong-Chien Lin in the Genetic Diversity Laboratory of National Taiwan Normal University for her help with the lab work. This study was funded by the Taiwan Forest Bureau, Council of Agriculture, ROC (95AS-11.1.3-FB-e1) to L-L Lee and supported by Taipei Zoo to C–C Shih.

PY - 2009/11

Y1 - 2009/11

N2 - Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

AB - Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears.

KW - Formosan black bear

KW - Tailed primers

KW - Tetranucleotide microsatellite

KW - Ursus thibetanus

UR - https://www.scopus.com/pages/publications/71349083553

UR - https://www.scopus.com/pages/publications/71349083553#tab=citedBy

U2 - 10.1007/s10592-009-9830-3

DO - 10.1007/s10592-009-9830-3

M3 - Comment/debate

AN - SCOPUS:71349083553

SN - 1566-0621

VL - 10

SP - 1845

EP - 1847

JO - Conservation Genetics

JF - Conservation Genetics

IS - 6

ER -

Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus

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