1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

Sheng Chia Chen; Chi Fon Chang; Pei Ju Fan; Ya Hsin Cheng; Tsunai Yu; Tai Huang Huang

doi:10.1007/s12104-012-9383-0

¹H, ¹³C and ¹⁵N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

Sheng Chia Chen, Chi Fon Chang, Pei Ju Fan, Ya Hsin Cheng, Tsunai Yu, Tai Huang Huang^*

^*此作品的通信作者

物理學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

2 引文斯高帕斯（Scopus）

摘要

Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the ¹H, ¹³C and ¹⁵N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

原文	英語
頁（從 - 到）	85-88
頁數	4
期刊	Biomolecular NMR Assignments
卷	7
發行號	1
DOIs	https://doi.org/10.1007/s12104-012-9383-0
出版狀態	已發佈 - 2013 4月

ASJC Scopus subject areas

結構生物學
生物化學

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10.1007/s12104-012-9383-0

其它文件與鏈接

Three-dimensional structure of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae
Li, Y. (Contributor), Chang, C. (Contributor), Chang, C. (Contributor), Cheng, Y. (Contributor), Fang, P. (Contributor), Yu, T. (Contributor), Chen, S. (Contributor), Li, Y. (Contributor), Hsiao, C. (Contributor), Huang, T. (Contributor) & Fan, P. (Contributor), Protein Data Bank (PDB), 2014 7月 16
DOI: 10.2210/pdb2MLK/pdb, https://www.wwpdb.org/pdb?id=pdb_00002mlk
資料集: Dataset

引用此

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title = "1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae",

abstract = "Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.",

keywords = "DNA-binding, Feo, Ferrous iron transport, Klebsiella pneumoniae, Two component systems",

author = "Chen, {Sheng Chia} and Chang, {Chi Fon} and Fan, {Pei Ju} and Cheng, {Ya Hsin} and Tsunai Yu and Huang, {Tai Huang}",

note = "Funding Information: Acknowledgments The NMR spectra were obtained at High-Field Nuclear Magnetic Resonance Center (HF-NMRC) supported by the National Research Program for Genomic Medicine. This work was supported by a grant from the National Science Council of the Republic of China (NSC 100-2311-B-001-023).",

year = "2013",

month = apr,

doi = "10.1007/s12104-012-9383-0",

language = "English",

volume = "7",

pages = "85--88",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Netherlands",

number = "1",

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TY - JOUR

T1 - 1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

AU - Chen, Sheng Chia

AU - Chang, Chi Fon

AU - Fan, Pei Ju

AU - Cheng, Ya Hsin

AU - Yu, Tsunai

AU - Huang, Tai Huang

N1 - Funding Information: Acknowledgments The NMR spectra were obtained at High-Field Nuclear Magnetic Resonance Center (HF-NMRC) supported by the National Research Program for Genomic Medicine. This work was supported by a grant from the National Science Council of the Republic of China (NSC 100-2311-B-001-023).

PY - 2013/4

Y1 - 2013/4

N2 - Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

AB - Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

KW - DNA-binding

KW - Feo

KW - Ferrous iron transport

KW - Klebsiella pneumoniae

KW - Two component systems

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1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

摘要

ASJC Scopus subject areas

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指紋

資料集

Three-dimensional structure of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

引用此

¹H, ¹³C and ¹⁵N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae