Study of the temperature dependent immuno-reaction kinetics for the bio-functionalized magnetic nanoparticle assay of bio-markers of colorectal cancer

Shieh-Yueh Yang*, J. F. Chang, T. C. Chen, C. C. Yang, C. S. Ho

*此作品的通信作者

    研究成果: 雜誌貢獻期刊論文同行評審

    5 引文 斯高帕斯(Scopus)

    摘要

    By conjugating antibodies on magnetic nanoparticles, target antigens can be quantitatively detected by measuring the magnetic signals of the magnetic nanoparticles due to their association with target antigens. This method of detection is called magnetically labeled immunoassay. The assay characteristics of magnetically labeled immunoassay have been reported widely. However, the immuno-reaction kinetics of magnetically labeled immunoassay has not been studied. In this work, the reaction rates between magnetic nanoparticles and target antigens are measured at various temperatures. It is found that the temperature dependent reaction rate obeys Arrheniuss equation, which shows the collision frequency and activation energy for the immuno-reaction between antibody-functionalized magnetic nanoparticles and target antigens. The carcinoembryonic antigen, which is a regular blood bio-marker for in-vitro diagnosis of colorectal cancer, is used as a target antigen for the example.

    原文英語
    文章編號013702
    期刊Applied Physics Letters
    104
    發行號1
    DOIs
    出版狀態已發佈 - 2014 一月 6

    ASJC Scopus subject areas

    • 物理與天文學(雜項)

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