Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh; J. J. Chieh; C. C. Yang; S. Y. Yang; Po Yu Chen; Yu Hao Huang; Y. W. Hong; H. E. Horng

doi:10.1016/j.jmmm.2012.08.029

Stability study for magnetic reagent assaying Hb and HbA1c

Wen Pin Hsieh, J. J. Chieh, C. C. Yang, S. Y. Yang, Po Yu Chen, Yu Hao Huang, Y. W. Hong, H. E. Horng^*

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe ₃O ₄ magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

原文	英語
頁（從 - 到）	147-150
頁數	4
期刊	Journal of Magnetism and Magnetic Materials
卷	326
DOIs	https://doi.org/10.1016/j.jmmm.2012.08.029
出版狀態	已發佈 - 2013 1月

ASJC Scopus subject areas

電子、光磁材料
凝聚態物理學

存取文件

10.1016/j.jmmm.2012.08.029

其它文件與鏈接

Link to publication in Scopus

引用此

@article{da1e47f90bb84ed591b7d0ace8a20b60,

title = "Stability study for magnetic reagent assaying Hb and HbA1c",

abstract = "Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.",

keywords = "Hb, HbA1c, Immunoassay, Magnetic particle, Stability",

author = "Hsieh, {Wen Pin} and Chieh, {J. J.} and Yang, {C. C.} and Yang, {S. Y.} and Chen, {Po Yu} and Huang, {Yu Hao} and Hong, {Y. W.} and Horng, {H. E.}",

note = "Funding Information: This work was supported by the National Science Council of Taiwan under Grant nos. NSC100-2112-M003-010 , NSC 100-2120-M-002-015, NSC101-2120-M168-001, and by the Department of Health under Grant nos. DOH100-TD-N-111-008 , DOH101-TD-N-111-004, and by the Ministry of Economic Affairs, Taiwan under Grant nos. 1Z1000408 , 1Z970688, 1Z0990415 (SBIR), and S09800226-203 (JAID).",

year = "2013",

month = jan,

doi = "10.1016/j.jmmm.2012.08.029",

language = "English",

volume = "326",

pages = "147--150",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier",

}

TY - JOUR

T1 - Stability study for magnetic reagent assaying Hb and HbA1c

AU - Hsieh, Wen Pin

AU - Chieh, J. J.

AU - Yang, C. C.

AU - Yang, S. Y.

AU - Chen, Po Yu

AU - Huang, Yu Hao

AU - Hong, Y. W.

AU - Horng, H. E.

N1 - Funding Information: This work was supported by the National Science Council of Taiwan under Grant nos. NSC100-2112-M003-010 , NSC 100-2120-M-002-015, NSC101-2120-M168-001, and by the Department of Health under Grant nos. DOH100-TD-N-111-008 , DOH101-TD-N-111-004, and by the Ministry of Economic Affairs, Taiwan under Grant nos. 1Z1000408 , 1Z970688, 1Z0990415 (SBIR), and S09800226-203 (JAID).

PY - 2013/1

Y1 - 2013/1

N2 - Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

AB - Reagents for magnetically labeled immunoassay on human Hb and human HbA1c have been synthesized. The reagents consist of Fe 3O 4 magnetic particles biofunctionalized with antibodies against Hb and HbA1c. It has been demonstrated that the reagents can be applied to quantitatively detect Hb and HbA1c by using immunomagnetic reduction assay. In addition to characterizing the assay properties, such as the standard curve and the low-detection limit, the stability of reagents is investigated. To do this, the temporal dependence of particle sizes and the bio-activity of reagents are monitored. The results show that the reagents are highly stable when stored at 2-8 °C. This means that the reagents synthesized in this work are promising for practical applications.

KW - Hb

KW - HbA1c

KW - Immunoassay

KW - Magnetic particle

KW - Stability

UR - http://www.scopus.com/inward/record.url?scp=84868676759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868676759&partnerID=8YFLogxK

U2 - 10.1016/j.jmmm.2012.08.029

DO - 10.1016/j.jmmm.2012.08.029

M3 - Article

AN - SCOPUS:84868676759

SN - 0304-8853

VL - 326

SP - 147

EP - 150

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

ER -

Stability study for magnetic reagent assaying Hb and HbA1c

摘要

ASJC Scopus subject areas

存取文件

其它文件與鏈接

指紋

引用此