Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Shih Hsien Hsu; Huey Wen Shyu; Hsiu Mei Hsieh-Li; Hung Li

doi:10.1016/S0925-4773(00)00513-X

Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Shih Hsien Hsu, Huey Wen Shyu, Hsiu Mei Hsieh-Li, Hung Li^*

^*此作品的通信作者

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

39 引文斯高帕斯（Scopus）

摘要

We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

原文	英語
頁（從 - 到）	177-187
頁數	11
期刊	Mechanisms of Development
卷	100
發行號	2
DOIs	https://doi.org/10.1016/S0925-4773(00)00513-X
出版狀態	已發佈 - 2001
對外發佈	是

ASJC Scopus subject areas

胚胎學
發展生物學

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10.1016/S0925-4773(00)00513-X

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title = "Spz1, a novel bHLH-Zip protein, is specifically expressed in testis",

abstract = "We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.",

keywords = "Basic helix-loop-helix, Leucine zipper, Mouse, Spermatogenesis, Testis",

author = "Hsu, {Shih Hsien} and Shyu, {Huey Wen} and Hsieh-Li, {Hsiu Mei} and Hung Li",

note = "Funding Information: We would like to thank Drs W.W. Branford, S.S. Potter and K.B. Choo for their critical reading of the manuscript. This work was supported in part by a research grant NSC-89-2311-B-001-214 from the National Science Council, Taiwan.",

year = "2001",

doi = "10.1016/S0925-4773(00)00513-X",

language = "English",

volume = "100",

pages = "177--187",

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T1 - Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

AU - Hsu, Shih Hsien

AU - Shyu, Huey Wen

AU - Hsieh-Li, Hsiu Mei

AU - Li, Hung

N1 - Funding Information: We would like to thank Drs W.W. Branford, S.S. Potter and K.B. Choo for their critical reading of the manuscript. This work was supported in part by a research grant NSC-89-2311-B-001-214 from the National Science Council, Taiwan.

PY - 2001

Y1 - 2001

N2 - We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

AB - We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

KW - Basic helix-loop-helix

KW - Leucine zipper

KW - Mouse

KW - Spermatogenesis

KW - Testis

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JO - Mechanisms of Development

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ER -

Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

摘要

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