Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

Shih Hsien Hsu, Huey Wen Shyu, Hsiu Mei Hsieh-Li, Hung Li

研究成果: 雜誌貢獻期刊論文同行評審

32 引文 斯高帕斯(Scopus)

摘要

We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules.

原文英語
頁(從 - 到)177-187
頁數11
期刊Mechanisms of Development
100
發行號2
DOIs
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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