Specific expression and regulation of glucose transporters in zebrafish ionocytes

Yung Che Tseng, Ruo Dong Chen, Jay Ron Lee, Sian Tai Liu, Shyh Jye Lee, Pung Pung Hwang

研究成果: 雜誌貢獻文章

47 引文 斯高帕斯(Scopus)

摘要

Glucose, a carbohydrate metabolite, plays a major role in the energy supply for fish iono- and osmoregulation, and the way that glucose is transported in ionocytes is a critical process related to the functional operations of ionocytes. Eighteen members of glucose transporters (GLUTs, SLC2A) were cloned and identified from zebrafish. Previously, Na+,K+-ATPase- rich (NaR), Na+-Cl- cotransporter-expressing (NCC), H +-ATPase-rich (HR), and glycogen-rich (GR) cells have been identified to be responsible for Ca2+ uptake, Cl- uptake, Na + uptake, and the energy deposition, respectively, in zebrafish skin/gills. The purpose of the present study was to test the hypothesis of whether GLUT isoforms are specifically expressed and function in ionocytes to supply energy for ion regulatory mechanisms. On the basis of translational knockdown of foxi3a/3b (2 transcriptional factors related to the ionocytes' differentiation) and triple in situ hybridization/ immunocytochemistry, 3 GLUT isoforms, zglut1a, -6, and -13.1, were specifically localized in NaR/NCC cells, GR cells, and HR cells, respectively. mRNA expression of zglut1a in embryos and adult gills were stimulated by the low Ca2+ or low Cl- freshwater, which has been previously reported to upregulate the functions (monitored by epithelial Ca2+ channel, NCC mRNA) of NaR/NCC cells, respectively while that of zglut13.1 was stimulated only by low Na+, a situation to upregulate the function (monitored by carbonic anhydrase 15a mRNA) of HR cells. On the other hand, ambient ion compositions did not affect the zglut6 mRNA expression. Taken together, zGLUT1a, -6, and 13.1, the specific transporters in NaR/NCC cells, GR cells, and HR cells, may absorb glucose into the respective cells to fulfill different physiological demands.

原文英語
頁(從 - 到)R275-R290
期刊American Journal of Physiology - Regulatory Integrative and Comparative Physiology
297
發行號2
DOIs
出版狀態已發佈 - 2009 八月 1

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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