SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice

Wei Fang Chang, Jie Xu, Chia Chun Chang, Shang Hsun Yang, Hsin Yang Li, Hsiu Mei Hsieh, Mong Hsun Tsai, Shinn Chih Wu, Winston T.K. Cheng, Ji Long Liu, Li Ying Sung

研究成果: 雜誌貢獻文章

7 引文 斯高帕斯(Scopus)

摘要

Survival motor neuron (SMN) is the determining factor in spinal muscular atrophy, the most common genetic cause of childhood mortality. We have previously found that SMN regulates stem cell division, proliferation and differentiation in Drosophila. However, it is unknown whether a similar effect exists in vertebrates. Here, we show that SMN is enriched in highly proliferative embryonic stem cells (ESCs) in mice and reduction of SMN impairs the pluripotency of ESCs. Moreover, we find that SMN reduction activates ERK signaling and affects neuronal differentiation in vitro. Teratomas with reduced SMN grow more slowly and show weaker signals of neuronal differentiation than those with a normal level of SMN. Finally, we show that over-expression of SMN is protective for ESCs from retinoic acid-induced differentiation. Taken together, our results suggest that SMN plays a role in the maintenance of pluripotent ESCs and neuronal differentiation in mice.

原文英語
頁(從 - 到)1539-1553
頁數15
期刊Brain Structure and Function
220
發行號3
DOIs
出版狀態已發佈 - 2015 五月 1

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology

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    Chang, W. F., Xu, J., Chang, C. C., Yang, S. H., Li, H. Y., Hsieh, H. M., Tsai, M. H., Wu, S. C., Cheng, W. T. K., Liu, J. L., & Sung, L. Y. (2015). SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice. Brain Structure and Function, 220(3), 1539-1553. https://doi.org/10.1007/s00429-014-0743-7