Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip

J. H. Chien, D. S. Lee, W. P. Chou, P. Y. Wang, Chii-Rong Yang, M. H. Wu, C. Y. Tsai, T. L. Chang, Y. W. Lee, Y. T. Cheng, P. H. Chen

研究成果: 書貢獻/報告類型會議貢獻

2 引文 斯高帕斯(Scopus)

摘要

The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1 C.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one.

原文英語
主出版物標題Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
頁面274-277
頁數4
DOIs
出版狀態已發佈 - 2006 十二月 1
事件1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS - Zhuhai, 中国
持續時間: 2006 一月 182006 一月 21

出版系列

名字Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS

其他

其他1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS
國家中国
城市Zhuhai
期間06/1/1806/1/21

ASJC Scopus subject areas

  • Biotechnology
  • Materials Science (miscellaneous)

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  • 引用此

    Chien, J. H., Lee, D. S., Chou, W. P., Wang, P. Y., Yang, C-R., Wu, M. H., Tsai, C. Y., Chang, T. L., Lee, Y. W., Cheng, Y. T., & Chen, P. H. (2006). Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip. 於 Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS (頁 274-277). [4134951] (Proceedings of 1st IEEE International Conference on Nano Micro Engineered and Molecular Systems, 1st IEEE-NEMS). https://doi.org/10.1109/NEMS.2006.334721