Sensitivity enhancement of fiber-optic biosensor by localized surface plasmon-coupled emission with gold nanoparticles

Ying Feng Chang*, Bao Yu Hsieh, Hsiang Yin Chen, Ming Yaw Ng, Wei Chih Liu, Yung Chang Chung, Hsieh Ting Wu, Chien Chou

*此作品的通信作者

研究成果: 書貢獻/報告類型會議論文篇章

2 引文 斯高帕斯(Scopus)

摘要

We proposed and developed a novel fiber-optic biosensor based on localized surface plasmon coupled emission (LSPCE) which consists of sandwich format of 〈antibody/antigen/Cy5-antibody-GNP〉 immuno-complex. It is immobilized on the surface of optical fiber where 〈Cy5-antibody-GNP〉 is a fluorescence probe produced by mixing Cy5 labeled antibody and protein A conjugated gold nanoparticles (Au-PA). The fluorophores are excited by localized surface plasmon (LSP) on gold nanoparticle (GNP) surface where the evanescent field is applied near the core surface of unclad optical fiber. Meanwhile, the fluorescence signal is detected by a photomultiplier tube being set beside the unclad optical fiber with high collection efficiency. In the experiment, this novel LSPCE biosensor demonstrates the minimum detectable concentration of mouse immunoglobulin G (IgG) at 1pg/ml (7fM) in the biomolecular interaction with anti-mouse IgG. From the experimental result, it verifies that LSPCE biosensor is a very high sensitive biosensor which is capable of measuring biomolecular interaction at very low concentration.

原文英語
主出版物標題Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs
出版狀態已發佈 - 2007 四月 30
事件Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, 美国
持續時間: 2007 一月 232007 一月 24

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6447
ISSN(列印)1605-7422

其他

其他Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
國家/地區美国
城市San Jose, CA
期間2007/01/232007/01/24

ASJC Scopus subject areas

  • 電子、光磁材料
  • 生物材料
  • 原子與分子物理與光學
  • 放射學、核子醫學和影像學

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