Sensitivity and kinetic analysis of graphene oxide-based surface plasmon resonance biosensors

Nan Fu Chiu*, Teng Yi Huang


研究成果: 雜誌貢獻期刊論文同行評審

75 引文 斯高帕斯(Scopus)


In this study, we demonstrate the fabrication of graphene oxide sheet (GOS)-based surface plasmon resonance (SPR) sensors for protein immobilization detection. GOS was used as the medium owing to its high covalent binding affinity for proteins. We were able to monitor the binding phenomenon in real time using the SPR-based technique. Thus, this GOS sensor can detect bovine albumin serum (BSA) directly in GOS films and exhibited up to a 4.3-fold higher angle shift than that of the conventional Au/Cr-based sensor in 10 ng/ml of BSA. The results showed that the SPR biosensor formed using a GOS solution of 2 mg/ml has optimal sensitive and had a BSA detection limit as low as 100 pg/ml. The sensitivity of the slope ratio of the GOS vs. conventional Au substrate is 2.4:1. Moreover, GOS is a label-free detection without additional amplification steps to enhance the sensitivity. In addition, the average value of the affinity binding constant KA for the GOS-based sensor with respect to BSA was 80.8 × 106 M-1. These results suggest that GOS-based SPR biosensors have potential for use in high-sensitivity protein immobilization detection.

頁(從 - 到)35-42
期刊Sensors and Actuators, B: Chemical
出版狀態已發佈 - 2014 7月 5

ASJC Scopus subject areas

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學


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