Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Chun Cheng Lin; Yi Chun Yeh; Chan Yi Yang; Chan Long Chen; Gee Fong Chen; Chia Chun Chen; Yi Chun Wu

doi:10.1021/ja0200903

Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Chun Cheng Lin^*, Yi Chun Yeh, Chan Yi Yang, Chan Long Chen, Gee Fong Chen, Chia Chun Chen, Yi Chun Wu

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

281 引文斯高帕斯（Scopus）

摘要

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

原文	英語
頁（從 - 到）	3508-3509
頁數	2
期刊	Journal of the American Chemical Society
卷	124
發行號	14
DOIs	https://doi.org/10.1021/ja0200903
出版狀態	已發佈 - 2002 4月 10

ASJC Scopus subject areas

催化
一般化學
生物化學
膠體和表面化學

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10.1021/ja0200903

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abstract = "The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.",

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AU - Lin, Chun Cheng

AU - Yeh, Yi Chun

AU - Yang, Chan Yi

AU - Chen, Chan Long

AU - Chen, Gee Fong

AU - Chen, Chia Chun

AU - Wu, Yi Chun

PY - 2002/4/10

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N2 - The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

AB - The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

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Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

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