Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli

Chun Cheng Lin*, Yi Chun Yeh, Chan Yi Yang, Chan Long Chen, Gee Fong Chen, Chia Chun Chen, Yi Chun Wu

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

263 引文 斯高帕斯(Scopus)

摘要

The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.

原文英語
頁(從 - 到)3508-3509
頁數2
期刊Journal of the American Chemical Society
124
發行號14
DOIs
出版狀態已發佈 - 2002 四月 10

ASJC Scopus subject areas

  • 催化
  • 化學 (全部)
  • 生物化學
  • 膠體和表面化學

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