摘要
In this study, a protein purified by fluorescein isothiocyanate (FITC)-affinity chromatography from human plasma was identified as albumin by MALDI-TOF-MS. Albumin was found to conjugate with FITC-labeled molecules through a copper-dependent reaction. The formation of this complex was confirmed by methods including a newly developed "charcoal-based fluorescence assay" (CFA), gel-filtration, affinity chromatography, and ultrafiltration. The binding was identified as disulfide bridge formation. This is the first to demonstrate that copper induces a covalent binding of FITC-labeled molecules with albumin. In addition, the developed CFA method facilitates the screening of small fluorescent dyes binding to macromolecules.
原文 | 英語 |
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頁(從 - 到) | 187-191 |
頁數 | 5 |
期刊 | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
卷 | 863 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 2008 2月 15 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 分析化學
- 生物化學
- 臨床生物化學
- 細胞生物學