SARS-CoV regulates immune function-related gene expression in human monocytic cells

Wanchung Hu, Yu Ting Yen, Sher Singh, Chuan Liang Kao, Betty A. Wu-Hsieh

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.

原文英語
頁(從 - 到)277-288
頁數12
期刊Viral Immunology
25
發行號4
DOIs
出版狀態已發佈 - 2012 八月 1

指紋

Severe Acute Respiratory Syndrome
Gene Expression
Monocytes
Coronavirus
Genes
Infection
Macrophages
Cathepsins
Hyperoxia
Cytokine Receptors
Pulmonary Fibrosis
Chemokine Receptors
Adult Respiratory Distress Syndrome
Proteasome Endopeptidase Complex
Lysosomes
Oligonucleotide Array Sequence Analysis
Transcriptome
Chemokines
Fibrosis
Down-Regulation

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

引用此文

SARS-CoV regulates immune function-related gene expression in human monocytic cells. / Hu, Wanchung; Yen, Yu Ting; Singh, Sher; Kao, Chuan Liang; Wu-Hsieh, Betty A.

於: Viral Immunology, 卷 25, 編號 4, 01.08.2012, p. 277-288.

研究成果: 雜誌貢獻文章

Hu, Wanchung ; Yen, Yu Ting ; Singh, Sher ; Kao, Chuan Liang ; Wu-Hsieh, Betty A. / SARS-CoV regulates immune function-related gene expression in human monocytic cells. 於: Viral Immunology. 2012 ; 卷 25, 編號 4. 頁 277-288.
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