Role of calcium-sensing receptor in mechanotransducer-channel-mediated Ca2+ influx in hair cells of zebrafish larvae

Li Yih Lin, Ya Hsin Yeh, Giun Yi Hung, Chia Hao Lin, Pung Pung Hwang, Jiun Lin Horng

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

The calcium-sensing receptor (CaSR) is an extracellular Ca2+ sensor that plays a critical role in maintaining Ca2+ homeostasis in several organs, including the parathyroid gland and kidneys. In this study, through in situ hybridization, the expression of CaSR mRNA was found in the neuromasts of zebrafish larvae. Immunohistochemistry further demonstrated that the CaSR protein was present in neuromast hair cell stereocilia and basolateral membranes. Based on the expression and subcellular localization of the CaSR in hair cells, we hypothesized that the CaSR is expressed in zebrafish lateral-line hair cells to regulate mechanotransducer (MET)-channel-mediated Ca2+ entry. Using the scanning ion-selective electrode technique, MET-channel-mediated Ca2+ influx at the stereocilia of hair cells was measured in intact larvae. Ca2+ influx was suppressed after larvae were pretreated with a CaSR activator (R-568) or high-Ca2+ (HCa) medium. Gene knockdown by using morpholino oligonucleotides decreased CaSR expression in hair cells and eliminated the effects of R-568 and HCa on Ca2+ influx. In addition, we found that treatment with R-568 attenuated neomycin-induced hair cell death. This study is the first to demonstrate that the CaSR is involved in mechanotransduction in zebrafish hair cells.

原文英語
文章編號649
期刊Frontiers in Physiology
9
發行號MAY
DOIs
出版狀態已發佈 - 2018 五月 30

指紋

Calcium-Sensing Receptors
Zebrafish
Larva
N-(2-chlorophenylpropyl)-1-(3-methoxyphenyl)ethylamine
Stereocilia
Gene Knockdown Techniques
Ion-Selective Electrodes
Morpholinos
Parathyroid Glands
Neomycin
In Situ Hybridization
Homeostasis
Cell Death
Immunohistochemistry
Kidney
Messenger RNA
Membranes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

引用此文

Role of calcium-sensing receptor in mechanotransducer-channel-mediated Ca2+ influx in hair cells of zebrafish larvae. / Lin, Li Yih; Yeh, Ya Hsin; Hung, Giun Yi; Lin, Chia Hao; Hwang, Pung Pung; Horng, Jiun Lin.

於: Frontiers in Physiology, 卷 9, 編號 MAY, 649, 30.05.2018.

研究成果: 雜誌貢獻文章

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abstract = "The calcium-sensing receptor (CaSR) is an extracellular Ca2+ sensor that plays a critical role in maintaining Ca2+ homeostasis in several organs, including the parathyroid gland and kidneys. In this study, through in situ hybridization, the expression of CaSR mRNA was found in the neuromasts of zebrafish larvae. Immunohistochemistry further demonstrated that the CaSR protein was present in neuromast hair cell stereocilia and basolateral membranes. Based on the expression and subcellular localization of the CaSR in hair cells, we hypothesized that the CaSR is expressed in zebrafish lateral-line hair cells to regulate mechanotransducer (MET)-channel-mediated Ca2+ entry. Using the scanning ion-selective electrode technique, MET-channel-mediated Ca2+ influx at the stereocilia of hair cells was measured in intact larvae. Ca2+ influx was suppressed after larvae were pretreated with a CaSR activator (R-568) or high-Ca2+ (HCa) medium. Gene knockdown by using morpholino oligonucleotides decreased CaSR expression in hair cells and eliminated the effects of R-568 and HCa on Ca2+ influx. In addition, we found that treatment with R-568 attenuated neomycin-induced hair cell death. This study is the first to demonstrate that the CaSR is involved in mechanotransduction in zebrafish hair cells.",
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AU - Hwang, Pung Pung

AU - Horng, Jiun Lin

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