RNA intrusions change DNA elastic properties and structure

Hsiang Chih Chiu, Kyung Duk Koh, Marina Evich, Annie L. Lesiak, Markus W. Germann*, Angelo Bongiorno, Elisa Riedo, Francesca Storici


研究成果: 雜誌貢獻期刊論文同行評審

30 引文 斯高帕斯(Scopus)


The units of RNA, termed ribonucleoside monophosphates (rNMPs), have been recently found as the most abundant defects present in DNA. Despite the relevance, it is largely unknown if and how rNMPs embedded in DNA can change the DNA structure and mechanical properties. Here, we report that rNMPs incorporated in DNA can change the elastic properties of DNA. Atomic force microscopy (AFM)-based single molecule elasticity measurements show that rNMP intrusions in short DNA duplexes can decrease-by 32%-or slightly increase the stretch modulus of DNA molecules for two sequences reported in this study. Molecular dynamics simulations and nuclear magnetic resonance spectroscopy identify a series of significant local structural alterations of DNA containing embedded rNMPs, especially at the rNMPs and nucleotide 3′ to the rNMP sites. The demonstrated ability of rNMPs to locally alter DNA mechanical properties and structure may help in understanding how such intrusions impact DNA biological functions and find applications in structural DNA and RNA nanotechnology.

頁(從 - 到)10009-10017
出版狀態已發佈 - 2014 九月 7

ASJC Scopus subject areas

  • 材料科學(全部)


深入研究「RNA intrusions change DNA elastic properties and structure」主題。共同形成了獨特的指紋。