Regulation of lactate dehydrogenase in tilapia (Oreochromis mossambicus) gills during acclimation to salinity challenge

Yung Che Tseng, Jay Ron Lee, Joshua Chia Hsi Chang, Chien Hsien Kuo, Shyh Jye Lee, Pung Pung Hwang*


研究成果: 雜誌貢獻期刊論文同行評審

24 引文 斯高帕斯(Scopus)


Upon salinity challenge, euryhaline teleosts immediately regulate the functions of many ion transporters and enzymes in gill mitochondrion-rich (MR) cells in order to maintain their internal homeostasis. A large amount of energy is necessary to operate these transporters and enzymes; however, the mechanism of energy metabolism in fish gills is still unclear. In the present study, tilapia (Oreochromis mossambicus) was used as a model animal to investigate the roles of lactate dehydrogenase (LDH) in the energy metabolism of fish gills during acclimation to seawater (SW) challenge. Results of LDH isozyme gel electrophoresis and Western blotting demonstrated that LDH1 is the major form and LDH5 the minor form expressed in tilapia gill epithelial cells. Immunocytochemical experiments indicated that both LDH1 and LDH5 were expressed in MR cells (using Na+-K+-ATPase as a marker). Protein expression levels of LDH1 and citrate synthase (CS) increased immediately in the 1st hour of acclimation to SW, while that of LDH5 was significantly enhanced during the 1st-3rd h. Taken together, an energy metabolism model in gill epithelial cells during SW acclimation is proposed: the tricarboxylic acid cycle in MR cells is stimulated to produce adenosine triphosphate (ATP) which initially triggers salt secretion; conversion of pyruvate to lactate is subsequently stimulated to enhance anaerobic respiration to produce additional energy.

頁(從 - 到)473-480
期刊Zoological Studies
出版狀態已發佈 - 2008 7月

ASJC Scopus subject areas

  • 動物科學與動物學


深入研究「Regulation of lactate dehydrogenase in tilapia (Oreochromis mossambicus) gills during acclimation to salinity challenge」主題。共同形成了獨特的指紋。