Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Yi Chieh Chou; Chia I. Shih; Chih Chia Chiang; Chun Hua Hsu; Yi Chun Yeh

doi:10.1016/j.snb.2019.126717

Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

Yi Chieh Chou
, Chia I. Shih
, Chih Chia Chiang
, Chun Hua Hsu
, Yi Chun Yeh^*

^*此作品的通信作者

化學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

35 引文斯高帕斯（Scopus）

摘要

As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

原文	英語
文章編號	126717
期刊	Sensors and Actuators, B: Chemical
卷	297
DOIs	https://doi.org/10.1016/j.snb.2019.126717
出版狀態	已發佈 - 2019 10月 15

ASJC Scopus subject areas

電子、光磁材料
儀器
凝聚態物理學
表面、塗料和薄膜
金屬和合金
電氣與電子工程
材料化學

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10.1016/j.snb.2019.126717

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title = "Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA",

abstract = "As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.",

keywords = "Catecholamine, Cell-based biosensor, Colorimetric biosensors, DOPA 4,5-Dioxygenase, L-DOPA",

author = "Chou, \{Yi Chieh\} and Shih, \{Chia I.\} and Chiang, \{Chih Chia\} and Hsu, \{Chun Hua\} and Yeh, \{Yi Chun\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = oct,

day = "15",

doi = "10.1016/j.snb.2019.126717",

language = "English",

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AU - Chou, Yi Chieh

AU - Shih, Chia I.

AU - Chiang, Chih Chia

AU - Hsu, Chun Hua

AU - Yeh, Yi Chun

PY - 2019/10/15

Y1 - 2019/10/15

N2 - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

AB - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.

KW - Catecholamine

KW - Cell-based biosensor

KW - Colorimetric biosensors

KW - DOPA 4,5-Dioxygenase

KW - L-DOPA

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UR - https://www.scopus.com/pages/publications/85067864776#tab=citedBy

U2 - 10.1016/j.snb.2019.126717

DO - 10.1016/j.snb.2019.126717

M3 - Article

AN - SCOPUS:85067864776

SN - 0925-4005

VL - 297

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

M1 - 126717

ER -

Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA

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ASJC Scopus subject areas

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