TY - JOUR
T1 - Reagent-free DOPA-dioxygenase colorimetric biosensor for selective detection of L-DOPA
AU - Chou, Yi Chieh
AU - Shih, Chia I.
AU - Chiang, Chih Chia
AU - Hsu, Chun Hua
AU - Yeh, Yi Chun
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.
AB - As the immediate precursor for the biosynthesis of dopamine (DA), L-DOPA remains a potent drug in the treatment of Parkinson's disease. In this study, we developed a novel DOPA-dioxygenase-based biosensor for the quantification of L-DOPA through the formation of yellow betalamic acid derivatives in vitro and in vivo. The proposed system is simple, rapid, sensitive, and selective to L-DOPA while remaining robust to interference from other catecholamines, and is further applicable for the determination of L-DOPA in spiked fetal bovine serum samples.
KW - Catecholamine
KW - Cell-based biosensor
KW - Colorimetric biosensors
KW - DOPA 4,5-Dioxygenase
KW - L-DOPA
UR - http://www.scopus.com/inward/record.url?scp=85067864776&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067864776&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.126717
DO - 10.1016/j.snb.2019.126717
M3 - Article
AN - SCOPUS:85067864776
SN - 0925-4005
VL - 297
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 126717
ER -