TY - JOUR
T1 - Rapid drug-screening and quantitation of 3,4-methylenedioxymethamphetamine in urine by MALDI-TOF mass spectrometry
AU - Su, An Kai
AU - Liu, Ju Tsung
AU - Lin, Cheng Huang
N1 - Funding Information:
This work was supported by a grant from the National Science Council of Taiwan under Contract No. NSC-92-2113-M-003-023. Permission was obtained from Pharmaceutical Affairs, Department of Health, Taiwan (License Number: ARR089000035).
PY - 2005/8/8
Y1 - 2005/8/8
N2 - A method for the rapid screening of the drugs, 3,4- methylenedioxymethamphetamine (MDMA), in urine by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. In this method, α-cyano-4-hydroxycinnamic acid (CHCA) is used as the matrix for the MALDI samples. The identity of MDMA was confirmed by flight time of the fragmentation ([M + H]+ ion) using a mass calculation curve, the quantification of MDMA was achieved using stable-isotope-labeled MDMA (MDMA-D5) as the internal standard. For the analysis of MDMA/MDMA-D5-spiked urine samples, the calibration curve was linear in the range of 0.1-100 ppm with a coefficient of determination, r2, of 0.9993. This assay was used to determine MDMA in actual urine samples. The MALDI-TOFMS results were comparable to the GC/MS results. No significant bias is shown between the two methods by t-test at 95% confidence level. We conclude that MALDI-TOFMS can serve as a reliable and complementary method to GC/MS for use in forensic analysis.
AB - A method for the rapid screening of the drugs, 3,4- methylenedioxymethamphetamine (MDMA), in urine by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. In this method, α-cyano-4-hydroxycinnamic acid (CHCA) is used as the matrix for the MALDI samples. The identity of MDMA was confirmed by flight time of the fragmentation ([M + H]+ ion) using a mass calculation curve, the quantification of MDMA was achieved using stable-isotope-labeled MDMA (MDMA-D5) as the internal standard. For the analysis of MDMA/MDMA-D5-spiked urine samples, the calibration curve was linear in the range of 0.1-100 ppm with a coefficient of determination, r2, of 0.9993. This assay was used to determine MDMA in actual urine samples. The MALDI-TOFMS results were comparable to the GC/MS results. No significant bias is shown between the two methods by t-test at 95% confidence level. We conclude that MALDI-TOFMS can serve as a reliable and complementary method to GC/MS for use in forensic analysis.
KW - α-Cyano-4-hydroxycinnamic acid (CHCA)
KW - 3,4-Methylenedioxymethamphetamine (MDMA)
KW - MALDI-TOF mass spectrometry
KW - Rapid drug-screening
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U2 - 10.1016/j.aca.2005.05.017
DO - 10.1016/j.aca.2005.05.017
M3 - Article
AN - SCOPUS:22044451104
SN - 0003-2670
VL - 546
SP - 193
EP - 198
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -