Protein Attachment on Nanodiamonds

Chung Lun Lin, Cheng Huang Lin, Huan Cheng Chang, Meng Chih Su

研究成果: 雜誌貢獻文章

16 引文 斯高帕斯(Scopus)

摘要

A recent advance in nanotechnology is the scale-up production of small and nonaggregated diamond nanoparticles suitable for biological applications. Using detonation nanodiamonds (NDs) with an average diameter of ∼4 nm as the adsorbents, we have studied the static attachment of three proteins (myoglobin, bovine serum albumin, and insulin) onto the nanoparticles by optical spectroscopy, mass spectrometry, and dynamic light scattering, and electrophoretic zeta potential measurements. Results show that the protein surface coverage is predominantly determined by the competition between protein-protein and protein-ND interactions, giving each protein a unique and characteristic structural configuration in its own complex. Specifically, both myoglobin and bovine serum albumin show a Langmuir-type adsorption behavior, forming 1:1 complexes at saturation, whereas insulin folds into a tightly bound multimer before adsorption. The markedly different adsorption patterns appear to be independent of the protein concentration and are closely related to the affinity of the individual proteins for the NDs. The present study provides a fundamental understanding for the use of NDs as a platform for nanomedical drug delivery.

原文英語
頁(從 - 到)7704-7711
頁數8
期刊Journal of Physical Chemistry A
119
發行號28
DOIs
出版狀態已發佈 - 2015 七月 16

    指紋

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

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