Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles

H. T. Chan; Y. Y. Do; P. L. Huang; P. L. Chien; T. S. Chan; R. S. Liu; C. Y. Huang; S. Y. Yang; H. E. Horng

doi:10.1016/j.jmmm.2006.01.126

Preparation and properties of bio-compatible magnetic Fe₃O₄ nanoparticles

H. T. Chan
, Y. Y. Do
, P. L. Huang^*
, P. L. Chien
, T. S. Chan
, R. S. Liu
, C. Y. Huang
, S. Y. Yang
, H. E. Horng

^*此作品的通信作者

光電工程研究所暨學士學位學程

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

53 引文斯高帕斯（Scopus）

摘要

In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe₃O₄) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

原文	英語
頁（從 - 到）	e415-e417
期刊	Journal of Magnetism and Magnetic Materials
卷	304
發行號	1
DOIs	https://doi.org/10.1016/j.jmmm.2006.01.126
出版狀態	已發佈 - 2006 9月

ASJC Scopus subject areas

電子、光磁材料
凝聚態物理學

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10.1016/j.jmmm.2006.01.126

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title = "Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles",

abstract = "In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.",

keywords = "Avidin, Bio-compatible nanoparticles, Biotinylated alkaline phosphatase, Hybridization",

author = "Chan, \{H. T.\} and Do, \{Y. Y.\} and Huang, \{P. L.\} and Chien, \{P. L.\} and Chan, \{T. S.\} and Liu, \{R. S.\} and Huang, \{C. Y.\} and Yang, \{S. Y.\} and Horng, \{H. E.\}",

note = "Funding Information: We thank the financial support from the Ministry Economic Affairs of Taiwan under the Grant number 94-EC-17-A-08-S1-0006. Research in P.L. Huang's laboratory relating to this work is supported by the Council of Agriculture, Republic of China (Grant Nos. 94AS-5.1.3-ST-a4 and 94AS-5.2.1-AD-U2).",

year = "2006",

month = sep,

doi = "10.1016/j.jmmm.2006.01.126",

language = "English",

volume = "304",

pages = "e415--e417",

journal = "Journal of Magnetism and Magnetic Materials",

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T1 - Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles

AU - Chan, H. T.

AU - Do, Y. Y.

AU - Huang, P. L.

AU - Chien, P. L.

AU - Chan, T. S.

AU - Liu, R. S.

AU - Huang, C. Y.

AU - Yang, S. Y.

AU - Horng, H. E.

N1 - Funding Information: We thank the financial support from the Ministry Economic Affairs of Taiwan under the Grant number 94-EC-17-A-08-S1-0006. Research in P.L. Huang's laboratory relating to this work is supported by the Council of Agriculture, Republic of China (Grant Nos. 94AS-5.1.3-ST-a4 and 94AS-5.2.1-AD-U2).

PY - 2006/9

Y1 - 2006/9

N2 - In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

AB - In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin.

KW - Avidin

KW - Bio-compatible nanoparticles

KW - Biotinylated alkaline phosphatase

KW - Hybridization

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UR - https://www.scopus.com/pages/publications/33646863030#tab=citedBy

U2 - 10.1016/j.jmmm.2006.01.126

DO - 10.1016/j.jmmm.2006.01.126

M3 - Article

AN - SCOPUS:33646863030

SN - 0304-8853

VL - 304

SP - e415-e417

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1

ER -

Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles

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ASJC Scopus subject areas

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Preparation and properties of bio-compatible magnetic Fe₃O₄ nanoparticles