Peptide-imprinted conductive polymer on continuous monolayer molybdenum disulfide transferred electrodes for electrochemical sensing of Matrix Metalloproteinase-1 in lung cancer culture medium

Mei Hwa Lee, Cheng Chih Lin, Wlodzimierz Kutner, James L. Thomas, Chu Yun Lin, Zofia Iskierko, Yu Seng Ku, Chien Yu Lin, Pawel Borowicz, Piyush Sindhu Sharma, Yann Wen Lan*, Chien Hsin Yang*, Hung Yin Lin*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

14 引文 斯高帕斯(Scopus)

摘要

Matrix metalloproteinase-1 (MMP-1) is associated with lung cancer, and thus the monitoring of the concentration of this protein may be useful for assessing lung cancer progression. In this work, poly(triphenylamine rhodanine-3-acetic acid-co-3,4-ethoxylene dioxy thiophene)s were incorporated onto a continuous monolayer (cML) of molybdenum disulfide (MoS2) by peptide-imprinted electropolymerization, in which peptides of MMP-1 were used as the templates in epitope imprinting. The morphology of the MoS2 thin film was monitored using optical and scanning electron microscopy. The imprinted MoS2 thin film electrode electrochemically responseded (at 1.0 pg/mL MMP-1) 1.4 times than ordinary imprinted electrodes. Moreover, MoS2 cML coating with PIPs can extend the sensing range of the PIP-coated electrodes from 1.0 pg/mL up to 10.0 pg/mL. The MoS2 cML electrode was used to determine that the MMP-1 concentration in the A549 cell line culture medium was 800 ng/mL. Comparison with results from a commercially available ELISA kit indicated an accuracy of 95 ± 5%.

原文英語
文章編號100258
期刊Biosensors and Bioelectronics: X
13
DOIs
出版狀態已發佈 - 2023 5月

ASJC Scopus subject areas

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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