Opto-mechanical analysis for confocal laser scanning microscope

Gao Wei Chang, Ming Jenq Twu, Yu Hsuan Lin, Chia Cheng Liao, Hung Zen Kuo

研究成果: 雜誌貢獻Conference article

1 引文 (Scopus)

摘要

The confocal imaging has become one of the most widely applied microscopic techniques in various fields, such as biotechnology, automation engineering, optical engineering, solid-state physics, metallurgy, integrated circuit inspection, etc. Confocal laser scanning microscopy (CLSM) is primarily based on the use of apertures in the detection path to provide the acquired three-dimensional images with satisfactory contrast and resolution. The major objective of this paper is to analyze the imaging performance of the confocal microscopes with varying opto-mechanical conditions. In this paper, the working principles of the one- and two-dimensional scanning mechanisms in the microscopic systems are first reviewed and verified by opto-mechanical simulations. Then, for the imaging performance, the tolerance to the fabrication and assembly of the optical components in conventional confocal microscopes is also investigated by simulations. The simulation results indicate the importance of eliminating the effects of stray light in the microscopic systems.

原文英語
文章編號587710
頁(從 - 到)1-9
頁數9
期刊Proceedings of SPIE - The International Society for Optical Engineering
5877
DOIs
出版狀態已發佈 - 2005 十二月 29
事件Optomechanics 2005 - San Diego, CA, 美国
持續時間: 2005 八月 32005 八月 4

指紋

Laser Scanning
Confocal
Microscope
Microscopes
microscopes
Scanning
Imaging techniques
scanning
Lasers
Imaging
engineering
Solid state physics
Stray light
lasers
Optical engineering
metallurgy
biotechnology
solid state physics
simulation
Metallurgy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

引用此文

Opto-mechanical analysis for confocal laser scanning microscope. / Chang, Gao Wei; Twu, Ming Jenq; Lin, Yu Hsuan; Liao, Chia Cheng; Kuo, Hung Zen.

於: Proceedings of SPIE - The International Society for Optical Engineering, 卷 5877, 587710, 29.12.2005, p. 1-9.

研究成果: 雜誌貢獻Conference article

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