Optimized growth kinetics of Pichia pastoris and recombinant Candida rugosa LIP1 production by RSM

Shu Wei Chang, Chwen Jen Shieh, Guan-Chiun Lee, Casimir C. Akoh, Jei Fu Shaw*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

8 引文 斯高帕斯(Scopus)

摘要

A predictive model for Pichia pastoris expression of highly active recombinant Candida rugosa LIP1 was developed by combining the Gompertz function and response surface methodology (RSM) to evaluate the effect of yeast extract concentration, glucose concentration, temperature, and pH on specific responses. Each of the responses (maximum population densities, specific growth rate (μmax), protein concentration, and minimum lag phase duration) was determined using the modified Gompertz function. RSM and 4-factor-5-level central composite rotatable design (CCRD) were adopted to evaluate the effects of growth parameters, such as temperature (21.6-38.4°C), glucose concentration (0.3-3.7%), yeast extract (0.16-1.84%), and pH (5.3-8.7) on the responses of P. pastoris growth kinetics. Based on ridge maximum analysis, the optimum population density conditions were: temperature 24.4°C, glucose concentration 2.0%, yeast extract 1.5%, and pH 7.6. The optimum specific growth rate conditions were: temperature 28.9°C, glucose concentration 2.0%, yeast extract 1.1%, and pH 6.9. The optimum protein concentration conditions were: temperature 24.2°C, glucose concentration 1.9%, yeast extract 1.5%, and pH 7.6. Based on ridge minimum analysis, the minimal lag phase conditions were: temperature 32.3°C, glucose concentration 2.1%, yeast extract 1.1%, and pH 5.4. For the predicted value, the maximum population density, specific growth rate, protein concentration, and minimum lag phase duration were 15.7 mg/ml, 3.4 h-1, 0.78 mg/ml, and 4.2 h, and the actual values were 14.3 ± 3.5 mg/ml, 3.6 ± 0.6 h-1, 0.72 ± 0.2 mg/ml, and 4.4 ± 1.6 h, respectively.

原文英語
頁(從 - 到)28-40
頁數13
期刊Journal of Molecular Microbiology and Biotechnology
11
發行號1-2
DOIs
出版狀態已發佈 - 2006 七月 1

ASJC Scopus subject areas

  • 生物技術
  • 微生物學
  • 應用微生物與生物技術
  • 分子生物學

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