Optimal production of sulphuric acid by Thiobacillus thiooxidans using response surface methodology

Hsuan Liang Liu*, Yann Wen Lan, Yang Chu Cheng

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

117 引文 斯高帕斯(Scopus)

摘要

Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 24 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent variables, KH2PO4, (NH 4)2SO4, MgSO4 and elemental sulphur, in the growth medium were tested. Elemental sulphur was found to exhibit positive correlation with the sulphuric acid yield, whereas the other three variables showed no significant effect. No mutual interactions were found between elemental sulphur and each of the other three variables. The optimal composition of the growth medium to achieve the optimal production of sulphuric acid was determined as follows (g/l): KH2PO4=3.5, (NH 4)2SO4=4.9, MgSO4=0.7 and elemental sulphur=23.7. The corresponding sulphuric acid production and dry cell weight were 38 662 ppm and 0.7 g/l, which are about 2.6- and 1.75-fold increase, respectively, compared with those using the conventional T. thiooxidans optimum growth medium.

原文英語
頁(從 - 到)1953-1961
頁數9
期刊Process Biochemistry
39
發行號12
DOIs
出版狀態已發佈 - 2004 十月 29
對外發佈

ASJC Scopus subject areas

  • 生物工程
  • 生物化學
  • 應用微生物與生物技術

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