Optical mapping of transcriptional factor binding sites on single DNA molecules using nanofluidic devices

K. K. Sriram, Jia Wei Yeh, Yii Lih Lin, Yi-Ren Chang, Chia Fu Chou

研究成果: 書貢獻/報告類型會議貢獻

摘要

Transcriptional factors (TF) are proteins that bind to specific bases of DNA using DNA binding domains to carry out the process of transcription. Identification of TF binding sites is essential for understanding the regulatory circuits that control cellular processes such as cell division and differentiation as well as metabolic and physiological balance [1]. In this work, we deploy a proof-of-concept method adopting bio-conjugation, nanofluidic devices and single molecule techniques for direct mapping of TF binding sites on field stretched single DNA molecules. Here we demonstrated a simple and robust system for TF binding site identification, complementary to the existing techniques practiced in this field.

原文英語
主出版物標題Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
發行者Chemical and Biological Microsystems Society
頁面1873-1875
頁數3
ISBN(列印)9780979806452
出版狀態已發佈 - 2012 一月 1
事件16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, 日本
持續時間: 2012 十月 282012 十一月 1

其他

其他16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
國家日本
城市Okinawa
期間12/10/2812/11/1

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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