This study investigated the effects of four independent variables - text layout, switch position, topic of text, and luminance contrast of Chinese E-books - on reading time and reading accuracy. Forty-eight college students, 26 females and 22 males aged 18-23 years, were recruited for the experiment. The present study demonstrated that a down-to-up text direction with a switch located to the right was the best layout for Chinese E-books in terms of reading time and accuracy. Moreover, the present study also addressed the effects of interactions between text direction and luminance contrast on level of visual fatigue and between text direction and topic of text, between switch position and topic of text, and between text direction and switch position on reading time. The results of this study should contribute to research related to the impact of touch-pad or interface designs on reading, luminance contrast, interface layout, satisfaction, and reading comprehension.