Nonlinear bio-photonic crystal effects revealed with multimodal nonlinear microscopy

S. W. Chu, I. H. Chen, T. M. Liu, C. K. Sun*, S. P. Lee, B. L. Lin, P. C. Cheng, M. X. Kuo, D. J. Lin, H. L. Liu


研究成果: 雜誌貢獻期刊論文同行評審

97 引文 斯高帕斯(Scopus)


Highly optically active nonlinear bio-photonic crystalline and semicrystalline structures in living cells were studied by a novel multimodal nonlinear microscopy. Numerous biological structures, including stacked membranes and aligned protein structures are highly organized on a nanoscale and have been found to exhibit strong optical activities through second-harmonic generation (SHG) interactions, behaving similarly to man-made nonlinear photonic crystals. The microscopic technology used in this study is based on a combination of different imaging modes including SHG, third-harmonic generation, and multiphoton-induced fluorescence. With no energy release during harmonic generation processes, the nonlinear-photonic-crystal-like SHG activity is useful for investigating the dynamics of structure-function relationships at subcellular levels and is ideal for studying living cells, as minimal or no preparation is required.

頁(從 - 到)190-200
期刊Journal of Microscopy
出版狀態已發佈 - 2002 十二月

ASJC Scopus subject areas

  • 病理學與法醫學
  • 組織學


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