Neurotherapy of Yi-Gan-San, a Traditional Herbal Medicine, in an Alzheimer’s Disease Model of Drosophila melanogaster by Alleviating Aβ42 Expression

Ming Tsan Su, Yong Sin Jheng, Chen Wen Lu, Wen Jhen Wu, Shieh Yueh Yang, Wu Chang Chuang, Ming Chung Lee, Chung Hsin Wu*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

摘要

Alzheimer’s disease (AD), a main cause of dementia, is the most common neurodegenera-tive disease that is related to the abnormal accumulation of amyloid β (Aβ) proteins. Yi-Gan-San (YGS), a traditional herbal medicine, has been used for the management of neurodegenerative disorders and for the treatment of neurosis, insomnia and dementia. The aim of this study was to examine antioxidant capacity and cytotoxicity of YGS treatment by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays in vitro. We explored neuroprotective effects of YGS treatment in alleviating Aβ neurotoxicity of Drosophila melanogaster in vivo by comparing survival rate, climbing index, and Aβ expressions through retinal green fluorescent protein (GFP) expression, highly sensitive immunomagnetic reduction (IMR) and Western blotting assays. In the in vitro study, our results showed that scavenging activities of free radical and SH-SY5Y nerve cell viability were increased significantly (p < 0.01–0.05). In the in vivo study, Aβ42-expressing flies (Aβ42-GFP flies) and their WT flies (mCD8-GFP flies) were used as an animal model to examine the neurotherapeutic effects of YGS treatment. Our results showed that, in comparison with those Aβ42 flies under sham treatments, Aβ42 flies under YGS treatments showed a greater survival rate, better climbing speed, and lower Aβ42 aggregation in Drosophila brain tissue (p < 0.01). Our findings suggest that YGS should have a beneficial alternative therapy for AD and dementia via alleviating Aβ neurotoxicity in the brain tissue.

原文英語
文章編號572
期刊Plants
11
發行號4
DOIs
出版狀態已發佈 - 2022 2月 1
對外發佈

ASJC Scopus subject areas

  • 生態學、進化論、行為學與系統學
  • 生態學
  • 植物科學

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