Na+,K+,2CI--cotransporter: A novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus

Yu Ching Wu, Li Yih Lin, Tsung Han Lee*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

51 引文 斯高帕斯(Scopus)

摘要

Yu-Ching Wu, Li-Yih Lin and Tsung-Han Lee (2003) Na+,K +,2CI--cotransporter: a novel marker for identifying freshwater-and seawater-type mitochondria-rich cells in gills of the euryhaline tilapia, Oreochromis mossambicus. Zoological Studies 42(1): 186-192. The abundance and location of the branchial Na+,K+2CI --cotransporter (NKCC) were examined in freshwater-and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater-or seawater-type MR cells in euryhaline tilapia. http://www.sinica. edu.tw/zool/zoolstud/42.1/186.pdf.

原文英語
頁(從 - 到)186-192
頁數7
期刊Zoological Studies
42
發行號1
出版狀態已發佈 - 2003 1月
對外發佈

ASJC Scopus subject areas

  • 動物科學與動物學

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