TY - JOUR
T1 - Multiple epigenetic biomarkers for evaluation of students' academic performance
AU - Lee, Li Ching
AU - Su, Ming Tsan
AU - Cho, Ying Chun
AU - Lee-Chen, Guey Jen
AU - Yeh, Ting Kuang
AU - Chang, Chun Yen
N1 - Funding Information:
Ministry of Education (MOE) in Taiwan, Grant/ Award Number: 107JIE0202; Ministry of Science and Technology, Taiwan, Grant/Award Numbers: MOST 104-2511-S-003-043-MY3, MOST 105-2511-S-003-041, MOST 107-2511-H-003-010-MY3, MOST 107-2634-F-008-003; National Taiwan Normal University from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan
Funding Information:
The work in this study was supported by the National Science Council of Taiwan under contracts MOST 104-2511-S-003-043-MY3, MOST 105-2511-S-003-041, MOST 107-2511-H-003-010-MY3, MOST 107-2634-F-008-003, and this work was financially supported by the “Institute for Research Excellence in Learning Sciences” of National Taiwan Normal University from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan.
Publisher Copyright:
© 2019 John Wiley & Sons Ltd and International Behavioural and Neural Genetics Society
PY - 2019/6
Y1 - 2019/6
N2 - Several reports have shown that methyl CpG-binding protein 2 (MeCP2), brain-derived neurotrophic factor (BDNF), phospho-cAMP response element-binding protein (p-CREB) and microRNAs may be important in regulating academic performance because of their roles in neuropsychiatry and cognitive diseases. The first goal of this study was to explore the associations among MeCP2, BDNF, CREB and academic performance. This study also examined the pathway responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. Scores from the basic competency test, an annual national competitive entrance examination, were used to evaluate academic performance. Subjects' plasma RNA was extracted and analyzed. This study determined that participants in the higher academic performance group had a significant difference in MECP2 mRNA expression compared with the lower academic performance group. We then used neuronal human derived neuroblastoma cell line (SH-SY5Y) cells with inducible MeCP2 expression from a second copy of the gene as a gain-of-function model and found that MeCP2 overexpression positively affected p-CREB and BDNF expression initially. After negative feedback, the p-CREB and BDNF levels subsequently decreased. In the neuronal phenotype examination, we found a significant reduction in total outgrowth and branches in MeCP2-induced cells compared with noninduced cells. This work describes pathways that may be responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. These results may shed light on the development of promising clinical treatment strategies in the area of neuropsychological adjustment.
AB - Several reports have shown that methyl CpG-binding protein 2 (MeCP2), brain-derived neurotrophic factor (BDNF), phospho-cAMP response element-binding protein (p-CREB) and microRNAs may be important in regulating academic performance because of their roles in neuropsychiatry and cognitive diseases. The first goal of this study was to explore the associations among MeCP2, BDNF, CREB and academic performance. This study also examined the pathway responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. Scores from the basic competency test, an annual national competitive entrance examination, were used to evaluate academic performance. Subjects' plasma RNA was extracted and analyzed. This study determined that participants in the higher academic performance group had a significant difference in MECP2 mRNA expression compared with the lower academic performance group. We then used neuronal human derived neuroblastoma cell line (SH-SY5Y) cells with inducible MeCP2 expression from a second copy of the gene as a gain-of-function model and found that MeCP2 overexpression positively affected p-CREB and BDNF expression initially. After negative feedback, the p-CREB and BDNF levels subsequently decreased. In the neuronal phenotype examination, we found a significant reduction in total outgrowth and branches in MeCP2-induced cells compared with noninduced cells. This work describes pathways that may be responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. These results may shed light on the development of promising clinical treatment strategies in the area of neuropsychological adjustment.
KW - academic performance
KW - brain-derived neurotrophic factor (BDNF)
KW - methyl CpG-binding protein 2 (MeCP2)
KW - microRNAs
KW - phospho-cAMP response element-binding protein (p-CREB)
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U2 - 10.1111/gbb.12559
DO - 10.1111/gbb.12559
M3 - Article
C2 - 30806012
AN - SCOPUS:85066869535
SN - 1601-1848
VL - 18
JO - Genes, Brain and Behavior
JF - Genes, Brain and Behavior
IS - 5
M1 - e12559
ER -