Morusin induces apoptosis and suppresses NF-κB activity in human colorectal cancer HT-29 cells

Jenq Chang Lee, Shen Jeu Won, Chien Lin Chao, Feng Ling Wu, Hsiao Sheng Liu, Pin Ling, Chun Nan Lin, Chun Li Su*

*此作品的通信作者

研究成果: 雜誌貢獻期刊論文同行評審

71 引文 斯高帕斯(Scopus)

摘要

Morusin is a pure compound isolated from root bark of Morus australis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G1 phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-α, IKK-β and IκB-α, increased expression of IκB-α, and suppressed nuclear translocation of NF-κB and its DNA binding activity. Dephosphorylation of NF-κB upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging caspase inhibitor suppressed morusin-induced apoptosis. Therefore, the antitumor mechanism of morusin in HT-29 cells may be via activation of caspases and inhibition of NF-κB.

原文英語
頁(從 - 到)236-242
頁數7
期刊Biochemical and Biophysical Research Communications
372
發行號1
DOIs
出版狀態已發佈 - 2008 7月 18
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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